anti myosin heavy chain mhc antibody  (Developmental Studies Hybridoma Bank)

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Comparison of mRNA expression levels of SM-MHC (A), α-SMA (B), SM-calponin (C), S100A4 (D), F4/80 (E), IL-6 (F), MCP-1 (G), Ccl5 (H), TGF-β (I), and Col1a1 (J) between SKO mice with AAA and DKO mice without aneurysm formation. Real-time PCR shows no significant differences in the mRNA expression levels of α-SMA , SM-MHC , or SM-calponin between the two groups. The mRNA expression levels of S100A4 , F4/80 , MCP-1 , Ccl5 , TGF-β and Col1a1 were significantly lower in the DKO group, without aneurysm formation, than in the SKO group, with AAA. Each data point represents an individual mouse (biological replicate). The numbers of analyzed samples were as follows: SM-MHC (SKO n = 8, DKO n = 7), α-SMA (SKO n = 7, DKO n = 7), SM-calponin (SKO n = 8, DKO n = 7), S100A4 (SKO n = 8, DKO n = 7), F4/80 (SKO n = 6, DKO n = 7), IL-6 (SKO n = 7, DKO n = 7), MCP-1 (SKO n = 8, DKO n = 7), Ccl5 (SKO n = 7, DKO n = 7), TGF-β (SKO n = 8, DKO n = 7), and Col1a1 (SKO n = 7, DKO n = 7). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant in Kruskal-Wallis test.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Quantitative histopathological and immunohistochemical analysis of aortic tissues. Representative images of Alcian blue staining (A, B), SM-MHC immunostaining (D, E), Mac-2 immunostaining (G, H), S100A4 immunostaining (J, K), MCP-1 immunostaining (M, N), and Ccl5 immunostaining (P, Q) in the SKO and DKO groups. Representative images from the SKO and DKO groups are shown in the upper and middle rows, respectively, and the corresponding quantitative analyses are shown in the bottom row. The corresponding quantitative analyses are shown in panels C, F, I, L, O, and R, respectively. The stained areas were quantified using ImageJ. For Alcian blue, SM-MHC, S100A4, MCP-1, and Ccl5 staining, the positive staining areas were normalized to the medial area of the aortic wall. For Mac-2 staining, the positive area was normalized to the combined intimal and medial area because macrophage infiltration was frequently observed in both layers. Each dot represents the value obtained from a single analyzed section. Sections containing advanced aneurysmal lesions were excluded to avoid secondary changes, and samples with inadequate tissue orientation were also excluded from the analysis. The numbers of analyzed sections were as follows: Alcian blue staining (SKO n = 21, DKO n = 39), SM-MHC staining (SKO n = 20, DKO n = 35), Mac-2 staining (SKO n = 29, DKO n = 37), S100A4 staining (SKO n = 15, DKO n = 15), MCP-1 staining (SKO n = 14, DKO n = 16), and Ccl5 staining (SKO n = 14, DKO n = 16). Statistical comparisons between groups were performed using the Kruskal–Wallis test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal: Acta Pharmacologica Sinica

Article Title: The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure

doi: 10.1038/s41401-026-01827-4

Figure Lengend Snippet: Antibody information.

Article Snippet: β-MHC , A7564 , ABclonal, China.

Techniques:

Journal: Acta Pharmacologica Sinica

Article Title: The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure

doi: 10.1038/s41401-026-01827-4

Figure Lengend Snippet: a Representative echocardiographic images from sham or HF mice injected with AAV9-cTnT-vector (Vec) or AAV9-cTnT-SLCO5A1 (OE) via tail-vein injection. b Quantitative analysis of EF and FS ( n = 6). c Representative images of Masson’s trichrome staining of LV sections (scale bar = 1 mm). d Representative PSR staining images (scale bar = 1 mm). e , f Quantitative analysis of fibrosis and collagen areas from ( c , d ) ( n = 6). g , h Western blotting and quantitative analysis of cardiac fibronectin, collagen I, and α-SMA expression. i , j Western blotting and quantitative analysis of cardiac β-MHC, ANP, and BNP expression. k , l WGA staining and quantitative analysis. ** P < 0.01, *** P < 0.001, ## P < 0.01, and ### P < 0.001. Fibronectin, FN; Collagen I, Col.

Article Snippet: β-MHC , A7564 , ABclonal, China.

Techniques: Injection, Plasmid Preparation, Staining, Western Blot, Expressing

Journal: Acta Pharmacologica Sinica

Article Title: The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure

doi: 10.1038/s41401-026-01827-4

Figure Lengend Snippet: a Twenty-eight-day Kaplan‒Meier survival curves for sham or HF mice treated with NS or T1AM at the indicated doses ( n = 30 mice per group). b Representative echocardiographic images at 28 days post-MI ( n = 6). c Quantitative analysis of EF and FS. d Representative images of H&E-stained LV sections (scale bar = 50 μm). e Representative images following Masson’s trichrome staining (scale bar = 1 mm). f Representative images following PSR staining (scale bar = 1 mm). g Representative images following WGA staining of cross-sectional cardiac tissues (scale bar = 50 μm). h Representative images following immunofluorescence staining of α-SMA in cardiac sections (scale bar = 100 μm). i , j Quantitative analysis of fibrosis and collagen areas from ( e , f ) ( n = 6). k Quantitative analysis of the cardiomyocyte cross-sectional area from ( g ) ( n = 6). l Quantitative analysis of ɑ-SMA immunofluorescence staining data ( n = 6). m Representative Western blotting images showing cardiac Collagen I, Fibronectin, and α-SMA expression in mice 28 days post-MI. n Representative Western blotting images showing β-MHC, ANP, and BNP expression. o , p Quantitative analysis of the Western blotting results ( m , n ) ( n = 6). * P < 0.05. ** P < 0.01. *** P < 0.001, # P < 0.05. ## P < 0.01, and ### P < 0.001. Fibronectin, FN; Collagen I, Col.

Article Snippet: β-MHC , A7564 , ABclonal, China.

Techniques: Staining, Immunofluorescence, Western Blot, Expressing

Journal: Acta Pharmacologica Sinica

Article Title: The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure

doi: 10.1038/s41401-026-01827-4

Figure Lengend Snippet: a Experimental T1AM treatment regimen applied to ISO-PE-treated NMCMs. b , c Representative Western blotting images and quantitative analysis of SLCO5A1, β-MHC, ANP, and BNP expression in ISO-PE-induced NMCMs treated with vehicle or T1AM under the indicated conditions ( n = 6). d Experimental scheme for SLCO5A1 overexpression in ISO-PE-treated NMCMs. e , f Representative Western blot images and quantitative analysis of SLCO5A1, β-MHC, ANP, and BNP expression under the indicated conditions ( n = 6). ** P < 0.01, *** P < 0.001, # P < 0.05, ## P < 0.01, and ### P < 0.001.

Article Snippet: β-MHC , A7564 , ABclonal, China.

Techniques: Western Blot, Expressing, Over Expression

Journal: Acta Pharmacologica Sinica

Article Title: The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure

doi: 10.1038/s41401-026-01827-4

Figure Lengend Snippet: a Representative echocardiographic images of HF mice treated with NS (HF-NS), T1AM (HF-T1AM), T1AM with Neg (HF-T1AM+Neg), or SLCO5A1 knockdown shRNA (HF-T1AM + sh). b Quantitative analysis of EF and FS ( n = 6). c Representative images following Masson’s trichrome staining (scale bar = 1 mm). d Representative images following PSR staining (scale bar = 1 mm). e , f Quantitative analysis of the results in ( c ) and ( d ) ( n = 6). g Representative Western blot images showing cardiac Fibronectin, Collagen I, and α-SMA expression. h Representative Western blot images showing cardiac β-MHC, ANP and BNP expression. i , j Quantitative analysis of the results in ( g ) and ( h ) ( n = 6). k Experimental scheme used to combine T1AM treatment and SLCO5A1 knockdown in ISO-PE-treated NMCMs. l , m Representative images following immunofluorescence staining of α-actinin and quantitative analysis of cardiomyocyte size ( n = 6). Scale bar = 25 μm. n , o Representative Western blotting images and quantitative analysis of SLCO5A1, β-MHC, ANP, and BNP expression in NMCMs under the indicated conditions ( n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.05, ## P < 0.01, ### P < 0.001, and $$ P < 0.01. Fibronectin, FN; Collagen I, Col.

Article Snippet: β-MHC , A7564 , ABclonal, China.

Techniques: Knockdown, shRNA, Staining, Western Blot, Expressing, Immunofluorescence